Modifying Enzymes
New England Biolabs, Inc. Quick CIP – 5000 units
Quick CIP is a heat-labile recombinant version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source. Quick CIP nonspecifically catalyzes the dephosphorylation of 5 and 3 ends of DNA and RNA phosphomonoesters. It also hydrolyses ribo- and deoxyribonucleoside triphosphates (NTPs and dNTPs). Quick CIP is useful in many applications that require the dephosphorylation of DNA or RNA ends. In cloning, dephosphorylation prevents re-ligation of linearized plasmid DNA. The enzyme can quickly dephosphorylate 5 protruding, 5 recessed, and blunt ends in just 10 minutes. Quick CIP may also be used to degrade unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis.
Worthington Biochemical Corporation Ribonuclease A, Purified, 100mg
A lyophilized preparation equivalent to Code: RASE. Contains some aggregates as a result of lyophilization but shows no difference in specific activity. Store at 2-8°C. Protect from moisture. Ribonuclease A has a molecular weight of 13,700 daltons. It operates in an optimum pH range of 7.0-7.5.
Sigma Aldrich Fine Chemicals Biosciences Alpha-Amylase from human saliva | 9000-90-2 | MFCD00081319 | 1 KU
Alpha-Amylase from human saliva | 9000-90-2 | MFCD00081319 | 1 KU
United States Biological Corporation ZYMOLYASE 20T
Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Source: Arthrobactor luteus Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 20u/mg
New England Biolabs, Inc. PNGase A – 150 units
PNGase A cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and short complex oligosaccharides such as those found in plant and insect cells from N-linked glycoproteins and glycopeptides. PNGase A differs from PNGase F in that it cleaves N-linked glycans with or without (1,3)-linked core fucose residues.
New England Biolabs, Inc. α2-3,6,8,9 Neuraminidase A - 4000 units
a2-3,6,8,9 Neuraminidase A is a broad specificity sialidase, which cleaves linear and branched non-reducing terminal sialic acid residues from glycoproteins, glycopeptides, and oligosaccharides. It can be used for glycan analysis and characterization and intact glycoproteins.
STEMCELL Technologies DNase I, Size: 25 mg
Deoxyribonuclease I (DNase I) is an endonuclease consisting of a single glycosylated polypeptide chain with two disulfide bonds. DNase is often included in tissue dissociation protocols to digest DNA that has leaked into the dissociation medium as a result of cell damage. DNase I preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides in both single- and double-stranded DNA, yielding polynucleotides with 5’-phosphate and 3’-hydroxyl groups (Bernardi et al.). DNase I has been used for the digestion of human tissues such as microglia (Klegeris & McGeer), cartilage (Dunham & Koch), colon (Fukushima & Fiocchi), epithelium (Fukushima & Fiocchi), liver (Vatakis et al.), lung (Fujino et al.), neural (Fuja et al.), and stem cells (Kusuma et al.).
Sigma Aldrich Fine Chemicals Biosciences Carbonic Anhydrase Isozyme II from bovine erythrocytes lyophilized powder, ≥3,000 W-A units/mg protein | 9001-03-0 | MFCD00081467 | 5mg
Carbonic Anhydrase Isozyme II from bovine erythrocytes lyophilized powder, ≥3,000 W-A units/mg protein | 9001-03-0 | MFCD00081467 | 5mg
Worthington Biochemical Corporation DNASE 5 VIAL/BOX
A component of the Papain Dissociation System. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 0.5 ml of EBSS or equivalent yields a solution of 2000 units/ml of deoxyribonuclease (1 mg/ml).
Worthington Biochemical Corporation DNASE 1 100MG
Deoxyribonuclease I, Chromatographically purified, Equivalent to 1X crystallized Dnase, A lyophilized powder with glycine as a stabilizer, Store at 2 to 8degreeC, Pack Size:100mg, Source: Bovine Pancreas, min. activity: 2000U/mgKunitz dry w
Worthington Biochemical Corporation Trypsin Inhibitor, Soybean, Purified, 100mg
Source: Soybean. Animal Free/AF. Chromatographically purified as described by Frattali, V., and Steiner, R.: Biochem., 7, 521 (1968), modified by Worthington. A dialyzed, lyophilized powder. Purity >90% checked using SDS PAGE. Store at 2-8°C.
AVIDITY LLC BirA biotin-protein ligase bulk reaction kit
The BirA biotin-protein ligase adds d-biotin covalently to biotin-acceptor peptides/proteins via an ATP intermediate (biotinyl 5’-adenylate) in a highly efficient and targeted manner. This kit includes 300 µg of BirA enzyme for those who require larger scale reactions or more frequent use of the BirA biotinylation reaction in their work.
Sigma Aldrich Fine Chemicals Biosciences Creatine phosphate | 71519-72-7 | MFCD00150192 | 5 g
Creatine phosphate | Purity: 97% | Mol Wt: 327.14 | 71519-72-7 | MFCD00150192 | 5 g