Modifying Enzymes
Worthington Biochemical Corporation Elastase, Lyophilized, 100mg
Two times crystallized, (Code: ESL), supplied as a dialyzed, lyophilized powder. The enzyme should be 0.22 micron filtered after reconstitution and prior to use. Suitable for the isolation of Type II lung cells. Store at 2-8°C. Porcine pancreatic elastase has a molecular weight of 25.9 kDa, and a pH optimum of 8.5. While elastase will hydrolyze a wide variety of protein substrates, it is unique among proteases in its ability to hydrolyze native elastin, a substrate not attacked by trypsin, chymotrypsin or pepsin. Soybean trypsin inhibitor and kallikrein inhibitor suppress proteolytic but not elastolytic activity.
GENOVIS INC Glyserias, 2000 units
Glyserias is an enzyme that cleaves the Fc portion of IgG antibodies. It is produced by Genovis and sold in vials containing 2000 units of the enzyme. Glyserias works optimally at pH 5.5-6.5 and 37°C. It can cleave both native and recombinant IgGs of all subclasses in just 30 minutes. Glyserias enables antibody deglycosylation, antibody fragmentation, and Fc/Fab separation. It has applications in structural biology, effector function studies, mass spec analysis, and antibody drug development. Glyserias provides an easy and gentle method of IgG cleavage compared to traditional proteolytic enzymes.
LGC GENOMICS LLC (Lucigen) RNase I, 1000 U @ 10 U/LCompletely digest single-stranded RNA with this heat-labile, non-sequence specific RNase.
RNase I degrades single-stranded RNA to nucleoside 3 monophosphates via 2,3 cyclic monophosphate intermediates by cleaving between all dinucleotide pairs,2,3 unlike RNase A, which cleaves only after cytosine and uridine. In addition, the enzyme is completely inactivated by heating at 70C for 15 minutes, eliminating the requirement to remove the enzyme prior to many subsequent procedures.
Enzyme Research Laboratories Inc HUMAN FIBRINOGEN 1G
Human Fibrinogen that is plasminogen, vWF and Fibronectin depleted.
Accelagen TURBONUCLEASE 50 000 UNITS
TurboNuclease is a recombinant Serratia macescens endonuclease (coded by the same gene as Bezonase). TurboNuclease degrades both DNA and RNA to oligonucleotides. This product is in low endotoxin, free of protease activity, and >99% pure with exceptionally high specific activity.
NEXPRING HEALTH Hyaluronidase Solution, 5 x 1 mL
For the removal of cumulus cells surrounding freshly retrieved oocytes.
Worthington Biochemical Corporation VENOM PHOSPHODIESTERASE I
Purified by the method of Williams, Sung and Laskowski, JBC, 236, 1130 (1961). Further treated to inactivate contaminating 5'-nucleotidase activity according to Sulkowski and Laskowski: BBA, 240, 443 (1961). Lyophilized in vials.
New England Biolabs, Inc. T7 DNA Ligase – 100000 units
T7 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T7. It will catalyze the formation of a phosphodiester bond between adjacent 5' phosphate and 3' hydroxyl groups of duplex DNA. Cohesive end ligation and nick sealing can be efficiently catalyzed by T7 DNA Ligase. However, unlike T4 and T3 DNA Ligases, blunt end ligation is not efficiently catalyzed by T7 DNA Ligase. Addition of high concentrations of PEG 6000 [>= 20% (w/v)] to the reaction can force T7 DNA Ligase to have measurable activity. However, under typical reaction conditions blunt-end DNA ligation does not occur in the presence of T7 DNA Ligase, making it a good choice for applications in which blunt and cohesive ends of DNA are present but only the cohesive ends are to be joined. T7 DNA Ligase does not ligate blunt-end DNA fragments.
AVIDITY LLC BIOTIN PROT.LIGASE 40UG 2X20
Biotin Protein Ligase 2 vials of 20 microgram (40 microgram);Concentration: 1 mg/ml. Activity: 5000 Units/microgram
Worthington Biochemical Corporation CHYMOTRYPSIN ALPHA TLCK 100MG
Chymotrypsin, alpha, TLCK treated, Storage: 2degree to 8degreeC, Source: Bovine pancreas, Pack Size: 100mg.
Discovery Life Sciences HUMAN UGT1A1 0.5ML@5MG/ML
BD Gentest, Human UGT1A1, Regulatory Status is RUO, 0.5mL@5mg/mL